Coding

Part:BBa_K4855001:Design

Designed by: Slavil Peykov   Group: iGEM23_Bulgaria   (2023-10-11)


blaIMP-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed as a gBlock fragment and ordered from IDT. C-terminal 6xHis tag was added for purifications.


Source

This part was based on protein sequence IMP-1 (Q79MP6). The final sequence was codon-optimized for optimal expression in E. coli.

References