Coding
Part:BBa_K4855001:Design
Designed by: Slavil Peykov Group: iGEM23_Bulgaria (2023-10-11)
blaIMP-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed as a gBlock fragment and ordered from IDT. C-terminal 6xHis tag was added for purifications.
Source
This part was based on protein sequence IMP-1 (Q79MP6). The final sequence was codon-optimized for optimal expression in E. coli.